1BIT
THE CRYSTAL STRUCTURE OF ANIONIC SALMON TRYPSIN IN A SECOND CRYSTAL FORM
Experimental procedure
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 77.090, 82.330, 31.160 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.830 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.27200 * |
| RMSD bond length | 0.021 |
| RMSD bond angle | 0.035 * |
| Phasing software | X-PLOR |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.830 * |
| Total number of observations | 25917 * |
| Number of reflections | 15984 * |
| Completeness [%] | 88.2 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 0.7 (M) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | benzamidine | 60 (mM) | |
| 5 | 1 | reservoir | sodium citrate-phosphate | 50 (mM) |
