1BIR

RIBONUCLEASE T1, PHE 100 TO ALA MUTANT COMPLEXED WITH 2' GMP

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	49.200, 48.190, 40.160
Unit cell angles	90.00, 90.26, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.186
Rwork	0.186
R-free	0.24000
RMSD bond length	0.012 *
RMSD bond angle	1.835 *
Data reduction software	MOSFLM
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.800	1.820 *
Rmerge	0.047 *	0.056 *
Number of reflections	17890 *
Completeness [%]	99.7 *	100 *
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.2 *		used to seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	recombinant Phe 100 Ala RNase T1	10 (mg/ml)
2	1	drop	2'-GMP	1.25 (%(w/v))
3	1	drop	sodium acetate	25 (mM)
4	1	drop	calcium acetate	2.5 (mM)
5	1	reservoir	MPD	52.5 (%)