1BIJ
CROSSLINKED, DEOXY HUMAN HEMOGLOBIN A
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 300 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-07 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.300, 96.000, 101.500 |
| Unit cell angles | 90.00, 101.50, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1HHB |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.877 |
| Data reduction software | R-AXIS |
| Phasing software | X-PLOR (3.843) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.200 * | 2.810 |
| High resolution limit [Å] | 2.000 | 2.250 |
| Rmerge | 0.040 * | |
| Number of reflections | 53187 | |
| <I/σ(I)> | 2.02 | |
| Completeness [%] | 73.0 | 73.1 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7.1 * | 4 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG6000 | 17 (%) | |
| 2 | 1 | 1 | phosphate | 0.01 (M) |
