1BIC

CRYSTALLOGRAPHIC ANALYSIS OF THR-200-> HIS HUMAN CARBONIC ANHYDRASE II AND ITS COMPLEX WITH THE SUBSTRATE, HCO3-

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	42.700, 41.700, 73.000
Unit cell angles	90.00, 104.60, 90.00

Refinement procedure

Resolution	? - 1.900
R-factor	0.146
RMSD bond length	0.014
RMSD bond angle	2.700
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.070 *
Total number of observations	28334 *
Number of reflections	15298 *
Completeness [%]	77.0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	ammonium sulfate	2.3 (M)
2	1	2	Tris-H2SO4	50 (mM)
3	1	2	dithiothreitol	4 (mM)	can be replaced with methylmercuric chloride
4	1	2	enzyme