1BIA

THE E. COLI BIOTIN HOLOENZYME SYNTHETASE(SLASH)BIO REPRESSOR CRYSTAL STRUCTURE DELINEATES THE BIOTIN AND DNA-BINDING DOMAINS

Experimental procedure

Spacegroup name	P 43 21 2
Unit cell lengths	114.000, 114.000, 60.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 2.300
R-factor	0.19
RMSD bond length	0.013
RMSD bond angle	2.400
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.300 *
Rmerge	0.075 *
Completeness [%]	70.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	phosphate	0.1 (M)
3	1	drop	glycerol	5 (%)
4	1	reservoir	phosphate	2.0 (M)