1BHW
LOW TEMPERATURE MIDDLE RESOLUTION STRUCTURE OF XYLOSE ISOMERASE FROM MASC DATA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF |
| Synchrotron site | ESRF |
| Temperature [K] | 124 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-06 |
| Detector | MAR scanner 300 mm plate |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 141.910, 141.910, 227.480 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 4.100 |
| R-factor | 0.315 |
| Rwork | 0.315 |
| R-free | 0.32100 |
| Structure solution method | MOLECULAR REPLACEMENT, RIGID BODY REFINEMENT |
| Starting model (for MR) | 1xin |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 105.400 | 4.290 |
| High resolution limit [Å] | 4.120 | 4.120 |
| Rmerge | 0.037 | 0.041 |
| Total number of observations | 110358 * | |
| Number of reflections | 19684 | |
| <I/σ(I)> | 17.3 | 16.1 |
| Completeness [%] | 97.3 | 75 |
| Redundancy | 5.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | PROTEIN WAS CRYSTALLIZED FROM AMMONIUM SULPHATE |
