1BHQ
MAC-1 I DOMAIN CADMIUM COMPLEX
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 287 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-08 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 48.420, 122.390, 75.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.700 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| Structure solution method | ISOMORPHOUS TO KNOWN PDB ENTRY 1IDN |
| Starting model (for MR) | CD11B I DOMAIN METAL FREE PDB ENTRY 1IDN |
| RMSD bond length | 0.018 |
| RMSD bond angle | 0.036 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.910 |
| High resolution limit [Å] | 2.700 | 2.650 |
| Rmerge | 0.097 * | |
| Total number of observations | 26642 * | |
| Number of reflections | 8618 * | |
| <I/σ(I)> | 10.6 | 3.1 |
| Completeness [%] | 43.9 | 43.3 |
| Redundancy | 3.1 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | reservoir was mixed 1:1 with 3 microlitter protein * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20-30 (mg/ml) | |
| 2 | 1 | drop | HEPES | 50 (mM) | |
| 3 | 1 | drop | sodium azide | 0.025 (%) | |
| 4 | 1 | reservoir | PEG6000 | 20-24 (%) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) |
