1BHE
POLYGALACTURONASE FROM ERWINIA CAROTOVORA SSP. CAROTOVORA
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X31 |
| Synchrotron site | EMBL/DESY, Hamburg |
| Beamline | X31 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-10 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.229, 53.033, 103.093 |
| Unit cell angles | 90.00, 112.59, 90.00 |
Refinement procedure
| Resolution | 12.500 - 1.900 |
| R-factor | 0.198 * |
| Rwork | 0.198 |
| R-free | 0.23900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.021 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.970 | |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.038 | 0.114 |
| Number of reflections | 31103 | |
| <I/σ(I)> | 21 | 9 |
| Completeness [%] | 97.0 | 95.2 * |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 18% PEG 8000, 0.1M SODIUM CACODYLATE, PH 6.5, 0.2M MAGNESIUM ACETATE. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 | 18 (%) | |
| 2 | 1 | reservoir | magnesium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
| 4 | 1 | drop | protein | 5 (mg/ml) |
