1BH2
A326S MUTANT OF AN INHIBITORY ALPHA SUBUNIT
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-12 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 79.584, 79.584, 105.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 18.000 - 2.100 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gia |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.508 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 20438 | |
| <I/σ(I)> | 10.8 | 4.6 |
| Completeness [%] | 88.4 | 88.1 |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6 | 21 * | Coleman, D.E., (1994) Science, 265, 1405. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25-30 (mg/ml) | |
| 2 | 1 | drop | 0.3 (mM) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | drop | DTT | 10 (mM) | |
| 6 | 1 | drop | sodium acetate | 200 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 1.8-1.9 (M) |
