1BGF

STAT-4 N-DOMAIN

Experimental procedure

Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Spacegroup name	P 65 2 2
Unit cell lengths	79.500, 79.500, 84.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 * - 1.450
R-factor	0.188
Rwork	0.188
R-free	0.21600
RMSD bond length	0.015 *
RMSD bond angle	1.400 *
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.01)

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	1.450
Rmerge	0.072 *	0.192 *
Total number of observations	237647 *
Number of reflections	27594 *
<I/σ(I)>	19.6
Completeness [%]	96.4	91.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	Tris-HCl	0.1 (M)
3	1	reservoir	PEG4000	17 (%)
4	1	drop	protein	20 (mg/ml)