1BGB
ECORV ENDONUCLEASE COMPLEX WITH 5'-CGGGATATCCC DNA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-08-15 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.400, 50.200, 64.100 |
| Unit cell angles | 96.00, 109.00, 108.00 |
Refinement procedure
| Resolution | 6.000 - 2.000 |
| R-factor | 0.184 |
| Rwork | 0.184 |
| R-free | 0.24600 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.120 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.053 | 0.077 |
| Number of reflections | 25665 | |
| <I/σ(I)> | 10 | 2.8 |
| Completeness [%] | 85.0 | 71 |
| Redundancy | 2.2 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.5 | CRYSTALLIZED FROM 15% PEG 4K, 100 MM IMIDAZOLE, (PH 6.5), 150 MM NACL., VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | IMIDAZOLE | ||
| 2 | 1 | 1 | NACL | ||
| 3 | 1 | 1 | PEG 4000 | ||
| 4 | 1 | 2 | PEG 4000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | EcoRV | 0.17 (mM) | |
| 2 | 1 | drop | DNA | 0.34 (mM) | |
| 3 | 1 | reservoir | PEG4000 | 15 (%) | |
| 4 | 1 | reservoir | imidazol | 100 (mM) | pH6.5 |
| 5 | 1 | reservoir | 150 (mM) |
