1BGA

BETA-GLUCOSIDASE A FROM BACILLUS POLYMYXA

Experimental procedure

Source type	SYNCHROTRON
Source details	LURE
Synchrotron site	LURE
Temperature [K]	176
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	205.460, 205.460, 155.860
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.2
Rwork	0.200
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cbg
RMSD bond length	0.009
RMSD bond angle	24.000 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.843)
Refinement software	X-PLOR (3.843)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.540	2.460
High resolution limit [Å]	2.400	2.400
Rmerge	0.083 *	0.300
Total number of observations	584536 *
Number of reflections	117344
<I/σ(I)>	8.3	1.8
Completeness [%]	97.3	92.3
Redundancy	4.7	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	co-crystallization *	8.3		PROTEIN WAS CRYSTALLIZED FROM 1.3 M NA/K PHOSPHATE, PH 8.3, 5 MICRO-L PROTEIN, 14 MG/ML, 5 MICRO-L RESERVOIR

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	phosphate	1.3 (M)
2	1	drop	inhibitor