1BG6

CRYSTAL STRUCTURE OF THE N-(1-D-CARBOXYLETHYL)-L-NORVALINE DEHYDROGENASE FROM ARTHROBACTER SP. STRAIN 1C

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1995-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	104.900, 80.000, 45.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.188 *
R-free	0.26300 *
Structure solution method	MIR
RMSD bond length	0.016
RMSD bond angle	17.470 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	CCP4
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	1.850
High resolution limit [Å]	1.800	1.800
Rmerge	0.040	0.152
Total number of observations	66518 *
Number of reflections	30854
<I/σ(I)>	8	5
Completeness [%]	89.0	78
Redundancy	2.3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6	290 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	potassium phosphate	0.1 (M)
3	1	reservoir	ammonium sulfate	36-37 (%sat)
4	1	reservoir	potassium phosphate	0.1 (M)