1BG6
CRYSTAL STRUCTURE OF THE N-(1-D-CARBOXYLETHYL)-L-NORVALINE DEHYDROGENASE FROM ARTHROBACTER SP. STRAIN 1C
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 290 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 104.900, 80.000, 45.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| R-factor | 0.188 * |
| R-free | 0.26300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 17.470 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((ROTAVATA)) |
| Phasing software | CCP4 |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 16.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.152 |
| Total number of observations | 66518 * | |
| Number of reflections | 30854 | |
| <I/σ(I)> | 8 | 5 |
| Completeness [%] | 89.0 | 78 |
| Redundancy | 2.3 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 290 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 0.1 (M) | |
| 3 | 1 | reservoir | ammonium sulfate | 36-37 (%sat) | |
| 4 | 1 | reservoir | potassium phosphate | 0.1 (M) |
