1BG0
TRANSITION STATE STRUCTURE OF ARGININE KINASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09-13 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.439, 70.885, 80.437 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.860 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MR, MIR |
| Starting model (for MR) | 1crk MITOCHONDRIAL CREATINE KINASE |
| RMSD bond length | 0.013 |
| RMSD bond angle | 22.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.940 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.047 | 0.157 |
| Number of reflections | 34737 | |
| <I/σ(I)> | 15.9 | |
| Completeness [%] | 98.9 | 98.5 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.5 | Zhou, G., (1997) Protein Sci., 6, 444. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG6000 | 12 (%) | can be replaced by PEG1000 |
