1BFT
STRUCTURE OF NF-KB P65 HOMODIMER BOUND TO A KB SITE
Experimental procedure
| Temperature [K] | 105 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-09 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 55.500, 74.490, 105.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.204 |
| R-free | 0.29400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | DIMERIZATION DOMAINS OF P50RHR/DNA COMPLEX |
| RMSD bond length | 0.014 |
| RMSD bond angle | 27.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.109 |
| Number of reflections | 12401 |
| <I/σ(I)> | 12.8 |
| Completeness [%] | 84.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG3350 | 25 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | ammonium acetate | 0.2 (M) |
