1BFD
BENZOYLFORMATE DECARBOXYLASE FROM PSEUDOMONAS PUTIDA
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 82.300, 96.600, 138.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.600 |
| R-factor | 0.15 * |
| Rwork | 0.152 |
| R-free | 0.18600 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.800 * |
| Data reduction software | PROCESS |
| Data scaling software | RIGAKU |
| Phasing software | PROTSYS |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 1.700 | |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.051 | |
| Total number of observations | 217078 * | |
| Number of reflections | 59179 | |
| <I/σ(I)> | 14 | 2.2 |
| Completeness [%] | 81.0 | 32 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 0.1 (mM) | ||
| 3 | 1 | drop | ThDP | 0.2 (mM) | |
| 4 | 1 | drop | NaHepes | 25 (mM) | |
| 5 | 1 | reservoir | PEG400 | 22 (%(v/v)) | |
| 6 | 1 | reservoir | 0.15 (M) | ||
| 7 | 1 | reservoir | MPD | 0.5 (%) | |
| 8 | 1 | reservoir | TrisCl | 0.1 (M) |
