1BFB

BASIC FIBROBLAST GROWTH FACTOR COMPLEXED WITH HEPARIN TETRAMER FRAGMENT

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	32.020, 41.800, 85.870
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 - 1.900
R-factor	0.206
Rwork	0.206
RMSD bond length	0.140
RMSD bond angle	2.350
Data reduction software	MSC
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	1.900
Rmerge	0.071 *
Total number of observations	23651 *
Number of reflections	9084
Completeness [%]	95.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	heparin tetrasaccharide	20 (mg/ml)
2	1	drop	bFGF	10 (mg/ml)
3	1	reservoir	PEG8000	25 (%)
4	1	reservoir	N-[2-acetamido]-2-iminodiacetic acid	0.1 (M)