1BEY

ANTIBODY TO CAMPATH-1H HUMANIZED FAB

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1992-12
Detector	MARRESEARCH
Spacegroup name	P 65 2 2
Unit cell lengths	80.060, 80.060, 347.250
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 3.250
R-factor	0.229 *
Rwork	0.214
R-free	0.30900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 7FAB (CON) 1FDL (VAR)
RMSD bond length	0.014
RMSD bond angle	25.200 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA))
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	3.420
High resolution limit [Å]	3.250	3.250
Rmerge	0.110	0.227 *
Number of reflections	10139
<I/σ(I)>	8	3.8
Completeness [%]	92.0	86.6 *
Redundancy	3	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7		pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG4000	20 (%)
3	1	reservoir	imidazole	0.2 (M)