1BEL
HYDROLASE PHOSPHORIC DIESTER, RNA
Experimental procedure
| Temperature [K] | 292 |
| Detector technology | IMAGE PLATE |
| Collection date | 1992-08-28 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.900, 44.800, 52.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.600 |
| R-factor | 0.15 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rn3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.925 |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | MERLOT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.800 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.083 | |
| Total number of observations | 76694 * | |
| Number of reflections | 12318 | |
| <I/σ(I)> | 6.36 | 4.22 |
| Completeness [%] | 82.0 | 61 |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.5 | 60% METHANOL, pH 4.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 15 (mg/ml) | |
| 2 | 1 | 1 | methanol | 58 (%(v/v)) |
