1BDN
CRYSTAL LATTICE PACKING IS IMPORTANT IN DETERMINING THE BEND OF A DNA DODECAMER CONTAINING AN ADENINE TRACT
Experimental procedure
| Temperature [K] | 285 |
| Detector technology | AREA DETECTOR |
| Detector | UCSD MARK II |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 24.540, 40.320, 65.860 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.201 |
| Refinement software | CORELS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 2267 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 10 * | pH 7.00, VAPOR DIFFUSION, HANGING DROP, temperature 283.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | MGCL2 | ||
| 4 | 1 | 1 | NA CACODYLATE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | DNA solution | 5 (mg/ml) | |
| 2 | 1 | reservoir | 44 (mM) | ||
| 3 | 1 | reservoir | sodium cacodylate | 10 (mM) | |
| 4 | 1 | reservoir | MPD | 40 (%(v/v)) |
