1BDJ
COMPLEX STRUCTURE OF HPT DOMAIN AND CHEY
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 283 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.175, 76.170, 83.270 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.680 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1A0B (HPT DOMAIN) AND 1CHN (CHEY) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 21.000 * |
| Data reduction software | WEIS |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 3.020 | |
| High resolution limit [Å] | 2.680 | 2.680 |
| Rmerge | 0.078 | 0.405 * |
| Total number of observations | 40201 * | |
| Number of reflections | 9392 | |
| <I/σ(I)> | 46.3 | 2.1 |
| Completeness [%] | 92.4 | 75.8 * |
| Redundancy | 4.4 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 277 * | Kato, M., (1998) Acta Crystallogr., D54, 140. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 30 (%) | |
| 2 | 1 | reservoir | ammonium sulfate | 200 (mM) | |
| 3 | 1 | drop | PEG4000 | 12 (%) | |
| 4 | 1 | drop | ammonium sulfate | 80 (mM) | |
| 5 | 1 | drop | protain | 12.8 (mg/ml) | |
| 6 | 1 | drop | 20 (mM) | ||
| 7 | 1 | drop | 10 (mM) |
