1BD9
HUMAN PHOSPHATIDYLETHANOLAMINE BINDING PROTEIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX7.2 |
| Synchrotron site | SRS |
| Beamline | PX7.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.420, 60.740, 67.640 |
| Unit cell angles | 90.00, 102.42, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.050 |
| R-factor | 0.171 * |
| Rwork | 0.171 |
| R-free | 0.23200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.024 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.140 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.058 | 0.120 |
| Number of reflections | 23495 | |
| <I/σ(I)> | 22.3 | 9.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 3.5 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 6.5 | PROTEIN WAS CRYSTALLIZED FROM 28-32% PEG 4000/6000/8000, 200-300 MM SODIUM ACETATE, 100MM SODIUM CACODYLATE, PH 6.5; THEN SOAKED IN 28-32% PEG 4000/6000/8000, 200-300 MM SODIUM ACETATE, 100MM BIS-TRIS, PH 6.5. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 5 (mg/ml) | can be replaced by 10mg/ml |
| 2 | 1 | 1 | Bis-Tris | 5 (mM) | |
| 3 | 1 | 1 | sodium azide | 0.01 (%) | |
| 4 | 1 | 2 | PEG4000 | 28-32 (%) | can be replaced by PEG6000 or PEG8000 |
| 5 | 1 | 2 | sodium acetate | 200-300 (mM) | |
| 6 | 1 | 2 | sodium azide | 0.01 (%) | |
| 7 | 1 | 2 | sodium cacodylate | 100 (mM) |
