1BD3

STRUCTURE OF THE APO URACIL PHOSPHORIBOSYLTRANSFERASE, 2 MUTANT C128V

Experimental procedure

Temperature [K]	298
Collection date	1997-08
Spacegroup name	P 1 21 1
Unit cell lengths	60.600, 141.690, 71.370
Unit cell angles	90.00, 115.13, 90.00

Refinement procedure

Resolution	10.000 - 1.930
R-factor	0.179 *
Rwork	0.186
RMSD bond length	0.014 *
RMSD bond angle	1.624
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.930	1.930 *
Rmerge	0.057 *
Total number of observations	178298 *
Number of reflections	75978 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)
2	1	drop	dithiothreitol	10 (mM)
3	1	drop	protein	25-40 (mg/ml)
4	1	reservoir	ammonium phosphate	1.0 (M)
5	1	reservoir	citrate	0.1 (M)
6	1	reservoir		0.2 (M)