1BCX

MUTATIONAL AND CRYSTALLOGRAPHIC ANALYSES OF THE ACTIVE SITE RESIDUES OF THE BACILLUS CIRCULANS XYLANASE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	44.090, 52.710, 78.970
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.810
R-factor	0.161
Rwork	0.161
RMSD bond length	0.008
RMSD bond angle	1.660
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.490 *
Rmerge	0.165 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			used to seed *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	Tris	40 (mM)
2	1	reservoir	ammonium sulfate	22 (%sat)
3	1	reservoir		100 (mM)
4	1	drop	protein	4 (mg/ml)