1BCP
BINARY COMPLEX OF PERTUSSIS TOXIN AND ATP
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 293 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1993-11-27 |
| Detector | WEISSENBERG |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 163.800, 98.200, 194.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.700 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.000 |
| Data reduction software | WEIS |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.500 | 2.500 * |
| Rmerge | 0.093 | |
| Total number of observations | 195544 * | |
| Number of reflections | 62637 | |
| Completeness [%] | 64.0 | 19.6 * |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | Stein, P.E., (1994) Structure (London), 2, 45. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium potassium phosphate | 25 (mM) | |
| 2 | 1 | reservoir | 0.25 (M) | ||
| 3 | 1 | reservoir | 0.02 (%) | ||
| 4 | 1 | reservoir | 0.3-0.5 (M) |
