1BC2
ZN-DEPENDENT METALLO-BETA-LACTAMASE FROM BACILLUS CEREUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 135 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 67.630, 67.630, 178.380 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.208 |
| Rwork | 0.208 |
| R-free | 0.27900 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 24.093 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | SOLOMON |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.000 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.054 | 0.289 |
| Number of reflections | 37917 | |
| <I/σ(I)> | 14.07 | 6.4 |
| Completeness [%] | 99.4 | 99.7 |
| Redundancy | 2.3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 18-20 * | HANGING DROP METHOD. PROTEIN WAS DISSOLVED IN 10MM TRIS PH7, 1MM ZINC SULPHATE. WELL SOLUTION CONTAINED 100MM TRIS-HCL PH4.5, 75% AMMONIUM SULPHATE. 6UL DROPS WERE MADE WITH 3UL PROTEIN SOLUTION 50% DILUTED (WATER) WELL SOLUTION., vapor diffusion - hanging drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2 (mg/ml) | |
| 2 | 1 | drop | 0.500 (mM) | ||
| 3 | 1 | drop | Tris-HCl | 10 (mM) | |
| 4 | 1 | drop | sodium azide | 0.125 (%(w/v)) | |
| 5 | 1 | reservoir | Tris-HCl | 10 (mM) | |
| 6 | 1 | reservoir | sodium azide | 0.125 (%(w/v)) | |
| 7 | 1 | reservoir | ammonium sulfate | 70-75 (%(v/v)sat) |
