1BBB

A THIRD QUATERNARY STRUCTURE OF HUMAN HEMOGLOBIN A AT 1.7-ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	97.500, 101.500, 61.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 1.700
R-factor	0.184
RMSD bond length	0.012
RMSD bond angle	2.500
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.700 *
Total number of observations	33563 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	protein solution	1 (%)
2	1	reservoir	cacodylate	100 (mM)	titrating sodium cacodylate with HCl to pH5.8
3	1	reservoir	PEG6000	16 (%)