1BAY
GLUTATHIONE S-TRANSFERASE YFYF CYS 47-CARBOXYMETHYLATED CLASS PI, FREE ENZYME
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-22 |
| Spacegroup name | I 4 |
| Unit cell lengths | 132.500, 132.500, 63.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.195 * |
| Rwork | 0.193 |
| R-free | 0.23600 |
| Structure solution method | MOLECULAR REEMPLACEMENT |
| Starting model (for MR) | 1glq |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.337 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.500 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | |
| Number of reflections | 31316 * | |
| Completeness [%] | 78.7 * | 56.8 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | HEPES | 17 (mM) | |
| 3 | 1 | drop | 32 (mM) | ||
| 4 | 1 | drop | PEG4000 | 4.2 (%) | |
| 5 | 1 | reservoir | PEG4000 | 19 (%) | |
| 6 | 1 | reservoir | 0.15 (M) | ||
| 7 | 1 | reservoir | HEPES | 75 (mM) |
