1BAW

PLASTOCYANIN FROM PHORMIDIUM LAMINOSUM

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1997-06
Detector	RIGAKU RAXIS
Spacegroup name	P 43 21 2
Unit cell lengths	86.570, 86.570, 91.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.199 *
Rwork	0.193
R-free	0.25200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.114 *	0.359 *
Number of reflections	8981 *	815 *
<I/σ(I)>	10	3
Completeness [%]	99.9	100
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	6	277 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	sodium cacodylate	0.1 (M)
2	1	1	zinc acetate	0.2 (M)
3	1	1	PEG8000	15 (%(v/v))