1BAO

THE CONTRIBUTION OF BURIED HYDROGEN BONDS TO PROTEIN STABILITY: THE CRYSTAL STRUCTURES OF TWO BARNASE MUTANTS

Experimental procedure

Spacegroup name	P 32
Unit cell lengths	58.809, 58.809, 81.902
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	6.000 - 2.200
R-factor	0.152
RMSD bond length	0.018
RMSD bond angle	0.053
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	2.200 *
Rmerge	0.172 *
Total number of observations	30016 *
Number of reflections	14908 *
Completeness [%]	92.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	reservoir	phosphate	2.8 (M)
3	1	reservoir		2 (mM)