1B9J

OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KLK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	110.045, 75.580, 70.455
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
Rwork	0.179
R-free	0.21900
Structure solution method	OTHER
Starting model (for MR)	1OLB
RMSD bond length	0.011
RMSD bond angle	0.028
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.039	0.048
Number of reflections	55029
<I/σ(I)>	13.1	5.3
Completeness [%]	90.9	55.6
Redundancy	17	8.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CO-CRYSTALLIZED WITH URANIUM ACETATE, PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG4000	5-10 (%(w/v))
3	1	reservoir	sodium acetate	50 (mM)
4	1	reservoir	uranyl acetate	1 (mM)