1B9B
TRIOSEPHOSPHATE ISOMERASE OF THERMOTOGA MARITIMA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 125.950, 125.950, 103.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.850 |
| R-factor | 0.211 * |
| Rwork | 0.211 |
| R-free | 0.24900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | BACILLUS STEAROTHERMOPHILUS TIM (PDB ENTRY 1BIM) |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.086 | 0.299 |
| Total number of observations | 88489 * | |
| Number of reflections | 22000 | |
| <I/σ(I)> | 9 | 6.7 |
| Completeness [%] | 97.8 | 90 |
| Redundancy | 3.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 70 (mM) | |
| 3 | 1 | drop | EDTA | 2 (mM) | |
| 4 | 1 | drop | cysteamine | 5 (mM) | |
| 5 | 1 | drop | 400 (mM) | ||
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
