1B98

NEUROTROPHIN 4 (HOMODIMER)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL1-5
Synchrotron site	SSRL
Beamline	BL1-5
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 1 21 1
Unit cell lengths	43.800, 50.800, 53.000
Unit cell angles	90.00, 109.40, 90.00

Refinement procedure

Resolution	20.000 - 2.350 *
R-factor	0.235
Rwork	0.235
R-free	0.33600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	NT4
RMSD bond length	0.015
RMSD bond angle	0.048
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.350 *
Rmerge	0.031
Number of reflections	7858
Completeness [%]	93.0
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	15 *	PEG 8000, PIPES, pH 6.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	10 (mM)
3	1	reservoir	PEG8000	22 (%)
4	1	reservoir	PIPES	100 (mM)
5	1	drop	24 amino acid peptide		1:2 ratio (NT4 protomer:peptide)