1B90

BACILLUS CEREUS BETA-AMYLASE APO FORM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	291
Detector technology	AREA DETECTOR
Collection date	1997-01
Detector	SIEMENS HI-STAR
Spacegroup name	P 1 21 1
Unit cell lengths	57.075, 92.103, 65.671
Unit cell angles	90.00, 101.81, 90.00

Refinement procedure

Resolution	10.000 - 2.500
Rwork	0.164
R-free	0.24700
Structure solution method	MIR
RMSD bond length	0.013
RMSD bond angle	24.250 *
Data reduction software	SAINT
Data scaling software	SAINT
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.578
High resolution limit [Å]	2.500	2.449
Rmerge	0.073 *
Total number of observations	59931 *
Number of reflections	24292
Completeness [%]	98.8	90
Redundancy	2.47

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6	18 *	drop consists of equal volume of protein and precipitant solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	acetate	0.1 (M)
3	1	reservoir	PEG6000	18 (%)	precipitant
4	1	reservoir	ammonium sulfate	5 (%sat)	precipitant
5	1	reservoir	acetate	0.1 (M)	precipitant