1B8R
PARVALBUMIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 28.850, 60.590, 54.480 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.1768 * |
| Rwork | 0.177 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpv AUTH A.L.SWAIN R.H.KRETSINGER E.L.AMMA |
| RMSD bond length | 0.012 |
| RMSD bond angle | 21.660 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 1.900 |
| Rmerge | 0.070 |
| Number of reflections | 6147 |
| Completeness [%] | 95.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | CRYSTALLIZATION CONDITIONS: PH 5.0 - 5.5, 3.1 - 3.2 M AMMONIUM SULFATE 0.05 - 0.10 M CACL2, ROOM TEMPERATURE, pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 0.05-0.10 (M) | ||
| 2 | 1 | reservoir | ammonium sulfate | 3.1-3.2 (M) |
