1B8L
Calcium-bound D51A/E101D/F102W Triple Mutant of Beta Carp Parvalbumin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-03 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.511, 37.298, 37.067 |
| Unit cell angles | 90.00, 113.12, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.700 |
| R-factor | 0.1678 |
| R-free | 0.22740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cpv AUTH A.L.SWAIN R.H.KRETSINGER E.L.AMMA |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.015 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.065 * |
| Number of reflections | 9239 |
| Completeness [%] | 94.0 * |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 * | 4 * | CRYSTALLIZATION CONDITIONS 40% PEG 4000, 50 MM CACL2, 50 M PH 7.0, 4 DEGREES C, pH 6.9 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 50 (mM) | |
| 2 | 1 | reservoir | 50 (mM) | ||
| 3 | 1 | reservoir | PEG4000 | 40 (%) |
