1B89

CLATHRIN HEAVY CHAIN PROXIMAL LEG SEGMENT (BOVINE)

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-06-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.9686,0.9794,0.9797,1.000
Spacegroup name	I 41 2 2
Unit cell lengths	145.200, 145.200, 166.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.600
R-factor	0.254
Rwork	0.254
R-free	0.27900
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	19.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.625
High resolution limit [Å]	2.500	2.500
Rmerge	0.050	0.137
Number of reflections	26124
<I/σ(I)>	21	4
Completeness [%]	71.5	39.5
Redundancy	7	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		250 MM SODIUM FORMATE, 100 MM TRIS-HCL, PH 8, pH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium formate	250 (mM)
2	1	reservoir	Tris-HCl	100 (mM)