1B88

V-ALPHA 2.6 MOUSE T CELL RECEPTOR (TCR) DOMAIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-06-15
Detector	RIGAKU RAXIS II
Spacegroup name	P 21 21 21
Unit cell lengths	53.987, 76.187, 88.402
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.500
R-factor	0.224 *
Rwork	0.224
R-free	0.32400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tcr
RMSD bond length	0.007
RMSD bond angle	26.890 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.600
High resolution limit [Å]	2.200	2.500
Rmerge	0.127
Total number of observations	174142 *
Number of reflections	13408
Completeness [%]	88.8	39.1
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7		SODIUM FORMATE, pH 7.0, VAPOR DIFFUSION, HANGING DROP

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	SODIUM FORMATE
2	1	2	SODIUM FORMATE