1B80

REC. LIGNIN PEROXIDASE H8 OXIDATIVELY PROCESSED

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM1A
Synchrotron site	ESRF
Beamline	BM1A
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1998-04
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	73.480, 94.930, 230.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.730
R-factor	0.17 *
Rwork	0.170
R-free	0.20800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1llp
RMSD bond length	0.012
RMSD bond angle	0.027
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.790
High resolution limit [Å]	1.730	1.730
Rmerge	0.078	0.270
Total number of observations	391636 *
Number of reflections	79369
<I/σ(I)>	12.2	4.4
Completeness [%]	94.4	91.1
Redundancy	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *	17 % PEG 6000 PH 3.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	sodium succinate	10 (mM)
3	1	reservoir	PEG6000	17 (%(w/v))