1B6V
CRYSTAL STRUCTURE OF A HYBRID BETWEEN RIBONUCLEASE A AND BOVINE SEMINAL RIBONUCLEASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 289 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.020, 75.660, 53.930 |
| Unit cell angles | 90.00, 101.15, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.25600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3rn3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 27.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.095 | |
| Total number of observations | 29658 * | |
| Number of reflections | 16566 | |
| <I/σ(I)> | 6.2 | |
| Completeness [%] | 96.5 | 89.7 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 * | 16 * | pH 4.3 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | PEG4000 | 10 (%(w/v)) | |
| 3 | 1 | drop | sodium citrate | 10 (mM) | |
| 4 | 1 | reservoir | PEG4000 | 20 (%(w/v)) | |
| 5 | 1 | reservoir | sodium citrate | 20 (mM) |
