1B6U
CRYSTAL STRUCTURE OF THE HUMAN KILLER CELL INHIBITORY RECEPTOR (KIR2DL3) SPECIFIC FOR HLA-CW3 RELATED ALLELES
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 288 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10 |
Detector | FUJI |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 95.400, 95.400, 130.800 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 15.000 - 3.000 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.32000 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.570 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (0.5) |
Refinement software | CNS (0.5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.195 | 0.490 |
Total number of observations | 18460 * | |
Number of reflections | 6281 | |
<I/σ(I)> | 4.7 | 1.5 |
Completeness [%] | 84.9 | 87.6 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, sitting drop * | 7.5 | 21 * | protein solution is mixed in a 1:1 ratio with well solution * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | reservoir | PEG8000 | 9-10 (%) | |
4 | 1 | reservoir | HEPES | 50 (mM) | |
5 | 1 | reservoir | ethylene glycol | 8 (%) | |
6 | 1 | reservoir | octyl-beta-glucoside | 0.5 (%) |