1B5S
DIHYDROLIPOYL TRANSACETYLASE (E.C.2.3.1.12) CATALYTIC DOMAIN (RESIDUES 184-425) FROM BACILLUS STEAROTHERMOPHILUS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-05 |
| Detector | FUJI |
| Spacegroup name | F 41 3 2 |
| Unit cell lengths | 534.400, 534.400, 534.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 4.400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1eaa |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.680 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.083 | 0.387 |
| Total number of observations | 219513 * | |
| Number of reflections | 34381 | |
| Completeness [%] | 63.4 | 34.9 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7 | pH 7 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | reservoir | MPD | 20 (%) | |
| 4 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 5 | 1 | reservoir | PEG1000 | 5 (%) |
