1B5Q
A 30 ANGSTROM U-SHAPED CATALYTIC TUNNEL IN THE CRYSTAL STRUCTURE OF POLYAMINE OXIDASE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 184.600, 184.600, 281.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 100.000 * - 1.900 |
R-free | 0.22900 * |
Structure solution method | MIR |
RMSD bond length | 0.011 |
RMSD bond angle | 2.260 |
Phasing software | CCP4 |
Refinement software | TNT |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 1.900 | |
Rmerge | 0.088 * | 0.173 * |
Total number of observations | 704940 * | |
Number of reflections | 211025 * | |
Completeness [%] | 96.6 * | 95.2 * |
Redundancy | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 6 * | 293 * | pH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | enzyme | 5 (mg/ml) | |
2 | 1 | drop | 300 (mM) | ||
3 | 1 | drop | sodium phosphate | 50 (mM) | |
4 | 1 | reservoir | ammonium sulfate | 42-48 (%sat) | |
5 | 1 | reservoir | 0.02 (%) | ||
6 | 1 | reservoir | sodium acetate | 100 (mM) |