1B59

COMPLEX OF HUMAN METHIONINE AMINOPEPTIDASE-2 COMPLEXED WITH OVALICIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F2
Synchrotron site	CHESS
Beamline	F2
Temperature [K]	100
Detector technology	CCD
Collection date	1998-08-15
Detector	ADSC
Spacegroup name	C 2 2 21
Unit cell lengths	89.723, 99.108, 101.292
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.178
Rwork	0.178
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bn5
RMSD bond length	0.012
RMSD bond angle	2.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	CNS (0.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.900
High resolution limit [Å]	1.800	1.800
Rmerge	0.074	0.298
Number of reflections	40120
<I/σ(I)>	15.2	3.9
Completeness [%]	96.3	97.7
Redundancy	5.1	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	5.4		15-30% T-BUTANOL, 50MM CITRATE BUFFER PH=5.2-5.4