1B57
CLASS II FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE IN COMPLEX WITH PHOSPHOGLYCOLOHYDROXAMATE
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 287 |
Detector technology | IMAGE PLATE |
Collection date | 1997-04 |
Detector | MARRESEARCH |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 78.237, 78.237, 289.687 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 30.000 - 2.000 |
Rwork | 0.192 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zen |
RMSD bond length | 0.013 |
RMSD bond angle | 0.032 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.224 |
Total number of observations | 55441 * | |
Number of reflections | 248236 | |
<I/σ(I)> | 16.7 | 1.4 |
Completeness [%] | 78.5 | 31.4 |
Redundancy | 5 | 1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 7.6 * | 4 * | pH 7.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | enzyme | 20 (mg/ml) | |
2 | 1 | 1 | Tris-HCl | 50 (mM) | |
3 | 1 | 1 | PGH | 10 (mM) |