1B4Z

OLIGO-PEPTIDE BINDING PROTEIN (OPPA) COMPLEXED WITH KDK

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	120
Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	110.010, 75.550, 70.381
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.700 *
Rwork	0.179
R-free	0.21400
Structure solution method	OTHER
Starting model (for MR)	1OLB
RMSD bond length	0.013
RMSD bond angle	0.029
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.800
High resolution limit [Å]	1.700 *	1.750
Rmerge	0.079
Number of reflections	59716
<I/σ(I)>	6.5
Completeness [%]	92.2	59.6
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5		CO-CRYSTALLIZED WITH URANIUM ACETATE, PH 5.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	reservoir	PEG4000	5-10 (%(w/v))
3	1	reservoir	sodium acetate	50 (mM)
4	1	reservoir	uranyl acetate	1 (mM)