1B4T

H48C YEAST CU(II)/ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298K) STRUCTURE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1996-01-15
Detector	MACSCIENCE
Spacegroup name	H 3 2
Unit cell lengths	118.647, 118.647, 75.376
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.209
Rwork	0.209
R-free	0.25800
Structure solution method	OTHER
Starting model (for MR)	1JCW
RMSD bond length	0.010
RMSD bond angle	1.500
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.080 *
Total number of observations	46156 *
Number of reflections	17197
<I/σ(I)>	11.8	2
Completeness [%]	90.7	75.8
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.7 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-25 (mg/ml)
2	1	1	phosphate	50 (mM)
3	1	2	ammonium sulfate	2.0-2.2 (M)
4	1	2	Tris-HCl	50 (mM)
5	1	2		50 (mM)
6	1	2		0.001 (%(w/v))