1B4J

COMPARISON OF THE THREE-DIMENSIONAL STRUCTURES OF A HUMANIZED AND A CHIMERIC FAB OF AN ANTI-GAMMA-INTERFERON ANTIBODY

Experimental procedure

Source type	ROTATING ANODE
Source details	SIEMENS
Temperature [K]	286
Detector technology	AREA DETECTOR
Collection date	1994-07
Detector	SIEMENS
Spacegroup name	P 21 21 21
Unit cell lengths	67.100, 75.800, 111.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.900
R-factor	0.193
Rwork	0.193
R-free	0.33000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dfb
RMSD bond length	0.009
RMSD bond angle	28.000 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.900
High resolution limit [Å]	2.730	2.730
Rmerge	0.097	0.560
Number of reflections	13866
<I/σ(I)>	6.7	0.62
Completeness [%]	87.9	0.45
Redundancy	2	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.2	18-20 *	pH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	35-45 (mg/ml)
2	1	reservoir	PEG8000	6.6 (%(w/v))
3	1	reservoir		0.05 (M)
4	1	reservoir	sodium azide	0.05 (%)