1B44
CRYSTAL STRUCTURE OF THE B SUBUNIT OF HEAT-LABILE ENTEROTOXIN FROM E. COLI CARRYING A PEPTIDE WITH ANTI-HSV ACTIVITY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-07-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 127.100, 127.100, 176.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 3.300 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.25500 |
| Structure solution method | OTHER |
| Starting model (for MR) | 1ltr CHAINS D-H |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | MOSFLM |
| Refinement software | X-PLOR (3.84) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.600 | 3.690 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.140 | 0.480 |
| Total number of observations | 64075 * | |
| Number of reflections | 16377 | 4728 * |
| <I/σ(I)> | 6.9 | 1.6 |
| Completeness [%] | 74.2 | 76.7 |
| Redundancy | 3.9 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 4 * | pH 6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7 (mg/ml) | |
| 2 | 1 | drop | lithium sulfate | 0.8 (M) | |
| 3 | 1 | drop | PEG8000 | 2 (%) | |
| 4 | 1 | reservoir | lithium sulfate | 0.8 (M) | |
| 5 | 1 | reservoir | PEG8000 | 2 (%) |
