1B3U
CRYSTAL STRUCTURE OF CONSTANT REGULATORY DOMAIN OF HUMAN PP2A, PR65ALPHA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-08 |
| Detector | ADSC QUANTUM |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.280, 112.150, 122.630 |
| Unit cell angles | 90.00, 95.60, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.300 |
| Rwork | 0.209 |
| R-free | 0.26800 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.006 |
| RMSD bond angle | 18.500 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((SCALA)) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.038 | 0.097 |
| Number of reflections | 63661 | 4473 * |
| <I/σ(I)> | 8.5 | 7.2 |
| Completeness [%] | 82.9 | 40.3 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.3 * | 20 * | pH 5.5 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | SODIUM CITRATE | ||
| 2 | 1 | 1 | PEG 4000 | ||
| 3 | 1 | 1 | NACL | ||
| 4 | 1 | 1 | DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 8 (%(w/v)) | |
| 2 | 1 | reservoir | sodium citrate | 100 (mM) |
